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R&D Systems goat polyclonal pd l2

FIGURE 1 | PD-L1, <t>PD-L2,</t> PD-1, CD8, and CD4 expression in p16-positive and p16-negative HNSCC. PD-L1, PD-L2, PD-1, CD8, and CD4 expression was assessed in tumor biopsy tissue from ﬁve p16-positive and four p16-negative HNSCC patients using immuno-histochemistry (details in Methods). Representative staining (scale bars, 100 µm) and cumulative data of marker expression (grading scale PD-L1/PD-L2: 1, low; 2, moderate; 3 high expression; grading scale PD-1, CD8, CD4: 1, <50 cells/ﬁeld; 2, 50–150 cells/ﬁeld; 3, >150 cells/ﬁeld).

Goat Polyclonal Pd L2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+polyclonal+pd+l2/Human+PD-L2%2FB7-DC+Antibody/pm31379843-69-42-47
Average 94 stars, based on 12 article reviews

goat polyclonal pd l2 - by Bioz Stars, 2026-10

94/100 stars

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1) Product Images from "TLR9 Mediated Tumor-Stroma Interactions in Human Papilloma Virus (HPV)-Positive Head and Neck Squamous Cell Carcinoma Up-Regulate PD-L1 and PD-L2."

Article Title: TLR9 Mediated Tumor-Stroma Interactions in Human Papilloma Virus (HPV)-Positive Head and Neck Squamous Cell Carcinoma Up-Regulate PD-L1 and PD-L2.

Journal: Frontiers in immunology

doi: 10.3389/fimmu.2019.01644

FIGURE 1 | PD-L1, PD-L2, PD-1, CD8, and CD4 expression in p16-positive and p16-negative HNSCC. PD-L1, PD-L2, PD-1, CD8, and CD4 expression was assessed in tumor biopsy tissue from ﬁve p16-positive and four p16-negative HNSCC patients using immuno-histochemistry (details in Methods). Representative staining (scale bars, 100 µm) and cumulative data of marker expression (grading scale PD-L1/PD-L2: 1, low; 2, moderate; 3 high expression; grading scale PD-1, CD8, CD4: 1, <50 cells/ﬁeld; 2, 50–150 cells/ﬁeld; 3, >150 cells/ﬁeld).

Figure Legend Snippet: FIGURE 1 | PD-L1, PD-L2, PD-1, CD8, and CD4 expression in p16-positive and p16-negative HNSCC. PD-L1, PD-L2, PD-1, CD8, and CD4 expression was assessed in tumor biopsy tissue from ﬁve p16-positive and four p16-negative HNSCC patients using immuno-histochemistry (details in Methods). Representative staining (scale bars, 100 µm) and cumulative data of marker expression (grading scale PD-L1/PD-L2: 1, low; 2, moderate; 3 high expression; grading scale PD-1, CD8, CD4: 1, <50 cells/ﬁeld; 2, 50–150 cells/ﬁeld; 3, >150 cells/ﬁeld).

Techniques Used: Expressing, Immunohistochemistry, Staining, Marker

FIGURE 3 | HPV-positive HNSCCs increase PD-L1 and PD-L2 on ﬁbroblasts. PD-L1 and PD-L2 expression on primary BJ human ﬁbroblasts, HPV-positive (SCC154), and HPV-negative (SCC099) HNSCC cell lines (HNSCCs) was detected by ﬂow cytometry. Illustrative histograms show PD-L1 and PD-L2 expression on ﬁbroblasts (black histograms), HPV-positive (red histograms), or HPV-negative (blue histograms) HNSCCs (A). Graphs show PD-L1 and PD-L2 expression (mean ± SEM; n = 5) in ﬁbroblasts, HPV-positive, and HPV-negative HNSCCs (B). Fibroblasts were cultured alone or co-cultured in direct contact (direct) with HPV-positive (SCC154) or HPV-negative (SCC099) HNSCCs. Fibroblasts were identiﬁed in co-cultures by lack of EpCAM expression. Illustrative histograms show PD-L1 and PD-L2 expression on ﬁbroblasts cultured alone (black histograms) or co-cultured directly with HPV-positive SCC154 (C; red histograms) or HPV-negative SCC099 (D; blue histograms). Graphs show PD-L1 and PD-L2 expression (mean ± SEM; n = 5) on ﬁbroblasts cultured alone (w/o) or co-cultured directly with HNSCC cells (E). HPV-positive (Continued)

Figure Legend Snippet: FIGURE 3 | HPV-positive HNSCCs increase PD-L1 and PD-L2 on ﬁbroblasts. PD-L1 and PD-L2 expression on primary BJ human ﬁbroblasts, HPV-positive (SCC154), and HPV-negative (SCC099) HNSCC cell lines (HNSCCs) was detected by ﬂow cytometry. Illustrative histograms show PD-L1 and PD-L2 expression on ﬁbroblasts (black histograms), HPV-positive (red histograms), or HPV-negative (blue histograms) HNSCCs (A). Graphs show PD-L1 and PD-L2 expression (mean ± SEM; n = 5) in ﬁbroblasts, HPV-positive, and HPV-negative HNSCCs (B). Fibroblasts were cultured alone or co-cultured in direct contact (direct) with HPV-positive (SCC154) or HPV-negative (SCC099) HNSCCs. Fibroblasts were identiﬁed in co-cultures by lack of EpCAM expression. Illustrative histograms show PD-L1 and PD-L2 expression on ﬁbroblasts cultured alone (black histograms) or co-cultured directly with HPV-positive SCC154 (C; red histograms) or HPV-negative SCC099 (D; blue histograms). Graphs show PD-L1 and PD-L2 expression (mean ± SEM; n = 5) on ﬁbroblasts cultured alone (w/o) or co-cultured directly with HNSCC cells (E). HPV-positive (Continued)

Techniques Used: Expressing, Cytometry, Cell Culture

FIGURE 4 | PD-L1 and PD-L2 expression in co-cultures of macrophages and HPV-positive or HPV-negative HNSCCs. Macrophages were cultured alone (w/o) or co-cultured in direct contact (direct) with HPV-positive (SCC154) or HPV-negative (SCC099) HNSCC cell lines (HNSCCs). Illustrative histograms show PD-L1 and PD-L2 expression on macrophages cultured alone (A; black histograms). Macrophages were identiﬁed in co-cultures by lack of EpCAM expression. Illustrative histograms show PD-L1 and PD-L2 expression on macrophages cultured alone (black histograms) or co-cultured directly with HPV-positive SCC154 (B; red histograms) or HPV-negative SCC099 (C; blue histograms). Graphs show PD-L1 and PD-L2 expression (mean ± SEM; n = 4) on macrophages cultured alone (w/o) or co-cultured directly with HNSCCs (D). HPV-positive (SCC154) or HPV-negative (SCC099) HNSCCs were cultured alone or co-cultured in direct contact (direct) with macrophages. HNSCCs were identiﬁed in co-cultures by EpCAM expression. Illustrative histograms show PD-L1 and PD-L2 expression on HPV-positive SCC154 cultured alone (black histograms) or co-cultured directly with macrophages (E; red histograms). Graphs show PD-L1 and PD-L2 expression (mean ± SEM; n = 3) on HPV-positive SCC154 cultured alone or co-cultured directly with macrophages (F). Illustrative histograms show PD-L1 and PD-L2 expression on HPV-negative SCC099 cultured alone (black histograms) or co-cultured directly with macrophages (G; blue histograms). Graphs show PD-L1 and PD-L2 expression (mean ± SEM; n = 3) on HPV-negative SCC099 cultured alone or co-cultured directly with macrophages (H). *p < 0.05; **p < 0.01; ns, not signiﬁcant (D: one-way ANOVA with Bonferroni correction for multiple comparisons; F,H: unpaired two-tailed Student’s t-test) Numbers adjacent to plots represent MFI values; dashed histograms show control staining with isotype-matched antibodies. MFI, mean ﬂuorescence intensity.

Figure Legend Snippet: FIGURE 4 | PD-L1 and PD-L2 expression in co-cultures of macrophages and HPV-positive or HPV-negative HNSCCs. Macrophages were cultured alone (w/o) or co-cultured in direct contact (direct) with HPV-positive (SCC154) or HPV-negative (SCC099) HNSCC cell lines (HNSCCs). Illustrative histograms show PD-L1 and PD-L2 expression on macrophages cultured alone (A; black histograms). Macrophages were identiﬁed in co-cultures by lack of EpCAM expression. Illustrative histograms show PD-L1 and PD-L2 expression on macrophages cultured alone (black histograms) or co-cultured directly with HPV-positive SCC154 (B; red histograms) or HPV-negative SCC099 (C; blue histograms). Graphs show PD-L1 and PD-L2 expression (mean ± SEM; n = 4) on macrophages cultured alone (w/o) or co-cultured directly with HNSCCs (D). HPV-positive (SCC154) or HPV-negative (SCC099) HNSCCs were cultured alone or co-cultured in direct contact (direct) with macrophages. HNSCCs were identiﬁed in co-cultures by EpCAM expression. Illustrative histograms show PD-L1 and PD-L2 expression on HPV-positive SCC154 cultured alone (black histograms) or co-cultured directly with macrophages (E; red histograms). Graphs show PD-L1 and PD-L2 expression (mean ± SEM; n = 3) on HPV-positive SCC154 cultured alone or co-cultured directly with macrophages (F). Illustrative histograms show PD-L1 and PD-L2 expression on HPV-negative SCC099 cultured alone (black histograms) or co-cultured directly with macrophages (G; blue histograms). Graphs show PD-L1 and PD-L2 expression (mean ± SEM; n = 3) on HPV-negative SCC099 cultured alone or co-cultured directly with macrophages (H). *p < 0.05; **p < 0.01; ns, not signiﬁcant (D: one-way ANOVA with Bonferroni correction for multiple comparisons; F,H: unpaired two-tailed Student’s t-test) Numbers adjacent to plots represent MFI values; dashed histograms show control staining with isotype-matched antibodies. MFI, mean ﬂuorescence intensity.

Techniques Used: Expressing, Cell Culture, Two Tailed Test, Control, Staining

FIGURE 5 | Conditioned medium from HPV-positive HNSCCs up-regulates PD-L1 and PD-L2 on ﬁbroblasts. Fibroblasts were cultured alone or co-cultured in direct contact with HPV-positive SCC154 (direct) or with conditioned medium from HPV-positive SCC154 (CM). Illustrative histograms show PD-L1 and PD-L2 expression on ﬁbroblasts cultured alone (black histograms) or co-cultured with conditioned medium from HPV-positive SCC154 (A; red histograms). Graphs show PD-L1 and PD-L2 expression (mean ± SEM; n = 13) on ﬁbroblasts cultured alone (w/o), co-cultured directly with HPV-positive SCC154 (direct) or with conditioned medium from HPV-positive SCC154 (CM) (B). HPV-positive (SCC154) HNSCCs were cultured alone or co-cultured in direct contact with ﬁbroblasts (Fibro direct) or with conditioned medium from ﬁbroblasts (Fibro CM). Illustrative histograms show PD-L1 and PD-L2 expression on HPV-positive SCC154 cultured alone (black histograms) or co-cultured with conditioned medium from ﬁbroblasts (C; red histograms). Graphs show PD-L1 and PD-L2 expression (mean ± SEM; n = 4) on HPV-positive SCC154 cultured alone (w/o), co-cultured directly with ﬁbroblasts (Fibro direct) or with conditioned medium from ﬁbroblasts (Fibro CM) (D). Macrophages were cultured alone (w/o) or co-cultured in direct contact with HPV-positive SCC154 (direct) or with conditioned medium from HPV-positive SCC154 (CM). Graphs show PD-L1 and PD-L2 expression (mean ± SEM; n = 3) on macrophages for the indicated conditions (E). HPV-positive SCC154 HNSCCs were cultured alone (w/o) or co-cultured in direct contact with macrophages (MFs direct) or with conditioned medium from macrophages (MFs CM). Graphs show PD-L1 and PD-L2 expression (mean ± SEM; n = 2) on HPV-positive SCC154 HNSCCs for the indicated conditions (F). **p < 0.01; ****p < 0.0001; ns, not signiﬁcant (one-way ANOVA with Bonferroni correction for multiple comparisons) Numbers adjacent to plots represent MFI values; dashed histograms show control staining with isotype-matched antibodies. MFI = mean ﬂuorescence intensity.

Figure Legend Snippet: FIGURE 5 | Conditioned medium from HPV-positive HNSCCs up-regulates PD-L1 and PD-L2 on ﬁbroblasts. Fibroblasts were cultured alone or co-cultured in direct contact with HPV-positive SCC154 (direct) or with conditioned medium from HPV-positive SCC154 (CM). Illustrative histograms show PD-L1 and PD-L2 expression on ﬁbroblasts cultured alone (black histograms) or co-cultured with conditioned medium from HPV-positive SCC154 (A; red histograms). Graphs show PD-L1 and PD-L2 expression (mean ± SEM; n = 13) on ﬁbroblasts cultured alone (w/o), co-cultured directly with HPV-positive SCC154 (direct) or with conditioned medium from HPV-positive SCC154 (CM) (B). HPV-positive (SCC154) HNSCCs were cultured alone or co-cultured in direct contact with ﬁbroblasts (Fibro direct) or with conditioned medium from ﬁbroblasts (Fibro CM). Illustrative histograms show PD-L1 and PD-L2 expression on HPV-positive SCC154 cultured alone (black histograms) or co-cultured with conditioned medium from ﬁbroblasts (C; red histograms). Graphs show PD-L1 and PD-L2 expression (mean ± SEM; n = 4) on HPV-positive SCC154 cultured alone (w/o), co-cultured directly with ﬁbroblasts (Fibro direct) or with conditioned medium from ﬁbroblasts (Fibro CM) (D). Macrophages were cultured alone (w/o) or co-cultured in direct contact with HPV-positive SCC154 (direct) or with conditioned medium from HPV-positive SCC154 (CM). Graphs show PD-L1 and PD-L2 expression (mean ± SEM; n = 3) on macrophages for the indicated conditions (E). HPV-positive SCC154 HNSCCs were cultured alone (w/o) or co-cultured in direct contact with macrophages (MFs direct) or with conditioned medium from macrophages (MFs CM). Graphs show PD-L1 and PD-L2 expression (mean ± SEM; n = 2) on HPV-positive SCC154 HNSCCs for the indicated conditions (F). **p < 0.01; ****p < 0.0001; ns, not signiﬁcant (one-way ANOVA with Bonferroni correction for multiple comparisons) Numbers adjacent to plots represent MFI values; dashed histograms show control staining with isotype-matched antibodies. MFI = mean ﬂuorescence intensity.

Techniques Used: Cell Culture, Expressing, Control, Staining

FIGURE 6 | Blockade of IFN-γ, TNF-α, or CD81 does not affect PD-L1 and PD-L2 up-regulation by HPV-positive HNSCCs. Fibroblasts were cultured alone (w/o) or co-cultured in direct contact with HPV-positive SCC154 (SCC154 direct) or with conditioned medium from HPV-positive SCC154 (SCC154 CM) as indicated. Graphs (A) show IFN-γ and TNF-α levels in culture supernatants (mean ± SEM; n = 4). The dashed red line indicates the lowest value (15.6 pg/ml) of the dynamic range for (Continued)

Figure Legend Snippet: FIGURE 6 | Blockade of IFN-γ, TNF-α, or CD81 does not affect PD-L1 and PD-L2 up-regulation by HPV-positive HNSCCs. Fibroblasts were cultured alone (w/o) or co-cultured in direct contact with HPV-positive SCC154 (SCC154 direct) or with conditioned medium from HPV-positive SCC154 (SCC154 CM) as indicated. Graphs (A) show IFN-γ and TNF-α levels in culture supernatants (mean ± SEM; n = 4). The dashed red line indicates the lowest value (15.6 pg/ml) of the dynamic range for (Continued)

Techniques Used: Cell Culture

Expressing:

Article Title: TLR9 Mediated Tumor-Stroma Interactions in Human Papilloma Virus (HPV)-Positive Head and Neck Squamous Cell Carcinoma Up-Regulate PD-L1 and PD-L2.
Article Snippet: The following primary antibodies were used: mouse monoclonal CD4 (#NCL-L-CD4-368); mouse monoclonal CD8 (#NCLL-CD8-4B11); mouse monoclonal CD68 (#NCL-L-CD68) and mouse monoclonal CD163 (#NCL-L-CD163) (all from Novocastra, Leica Biosystems); rabbit polyclonal anti-inducible nitric oxide synthase (iNOS, Abcam; #ab3523); goat polyclonal PD-1 (#AF1086); and goat polyclonal PD-L2 (#AF1224) (both R&D Systems); and mouse monoclonal CD274 (PD-L1) (Biolegend; #329702).. Paraffin sections of tumor tissue were cut at 4µm and heated for 45min at 60◦C prior to staining.Paraffin sections of tumor tissue were cut at 4µm and heated for 45min at 60◦C prior to staining.

Article Title: TLR9 Mediated Tumor-Stroma Interactions in Human Papilloma Virus (HPV)-Positive Head and Neck Squamous Cell Carcinoma Up-Regulate PD-L1 and PD-L2
Article Snippet: The following primary antibodies were used: mouse monoclonal CD4 (#NCL-L-CD4-368); mouse monoclonal CD8 (#NCL-L-CD8-4B11); mouse monoclonal CD68 (#NCL-L-CD68) and mouse monoclonal CD163 (#NCL-L-CD163) (all from Novocastra, Leica Biosystems); rabbit polyclonal anti-inducible nitric oxide synthase (iNOS, Abcam; #ab3523); goat polyclonal PD-1 (#AF1086); and goat polyclonal PD-L2 (#AF1224) (both R&D Systems); and mouse monoclonal CD274 (PD-L1) (Biolegend; #329702).. Paraffin sections of tumor tissue were cut at 4 μm and heated for 45 min at 60°C prior to staining.Paraffin sections of tumor tissue were cut at 4 μm and heated for 45 min at 60°C prior to staining.

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